e52a6f0149 View Article PubMed/NCBI Google Scholar 5. Performed the experiments: MP DR MMT KJW CR. View Article PubMed/NCBI Google Scholar 24. Antonicka H, Leary SC, Guercin GH, Agar JN, Horvath R, et al. JavaScript is disabled on your browser. J Appl Physiol 92: 24292438.
The antibodies recognize subunits proteins NDUFB8, CII-30, CIII-Core 2, COX-IV-1, and CV- of Complexes I, II, III, IV, and V (ATP synthase), respectively. Mol Cell Biochem 174: 5560. Mitochondrial H2O2 and was detected by measuring the rate of appearance of resorufin, which is produced from the reaction between H2O2 and Amplex Red, with a Hitachi F-2500 fluorescence spectrophotometer at an excitation/emission wavelength of 563/587 nm, using the FL-solutions software. HomeServicesContact . Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Interestingly, mitochondrial fission-induced fragmentation is a critical event facilitating the release of pro-apoptotic factors and mPTP opening [33], [34], [35]. To decline or learn more, visit our Cookies page.. Am J Physiol Cell Physiol 298: C13081314. In addition to concerns over selective and low yield of mitochondria via standard isolation procedures, other concerns relate to the disruption of mitochondrial three-dimensional network or reticular structure [25], [26] and lack of interaction with other cellular compartments (e.g., sarcoplasmic reticulum, cytoskeleton, lipid droplets) following the isolation of mitochondria [21], [23]. or its licensors or contributors.
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